• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

YY1 Monoclonal antibody, PBS Only

YY1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E11C5

Cat no : 66281-1-PBS

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using YY1 Monoclonal antibody (66281-1-Ig)

    WB analysis of HeLa using 66281-1-Ig (same clone as 66281-1-PBS)

    Western blot of YY1 in various human cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using YY1 Monoclonal antibody (66281-1-Ig)

    WB analysis of C6 using 66281-1-Ig (same clone as 66281-1-PBS)

    Western blot of YY1 in rat (C6 and ROS1728) and mouse(NIH3T3 and Raw264.7) cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using YY1 Monoclonal antibody (66281-1-Ig)

    WB analysis of MCF-7 using 66281-1-Ig (same clone as 66281-1-PBS)

    WB result of YY1 antibody (66281-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YY1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using YY1 Monoclonal antibody (66281-1-Ig)

    WB analysis using 66281-1-Ig (same clone as 66281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using YY1 Monoclonal antibody (66281-1-Ig)

    WB analysis of NIH/3T3 using 66281-1-Ig (same clone as 66281-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COS-7 cells using YY1 Monoclonal antibody (66281-1-Ig)

    WB analysis of COS-7 using 66281-1-Ig (same clone as 66281-1-PBS)

    COS-7 cells were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using YY1 Monoclonal antibody (66281-1-Ig)

    WB analysis of HeLa using 66281-1-Ig (same clone as 66281-1-PBS)

    Western blot of Hela cells with mouse anti-YY1 monoclonal antibody (66281-1-Ig) at various dilutions incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using YY1 Monoclonal antibody (66281-1-Ig)

    IP experiment of NIH/3T3 using 66281-1-Ig (same clone as 66281-1-PBS)

    IP result of anti-YY1 (IP:66281-1-Ig, 5ug; Detection:66281-1-Ig 1:1000) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using YY1 Monoclonal antibody (66281-1-Ig)

    IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using YY1 Monoclonal antibody (66281-1-Ig)

    IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YY1 Monoclonal antibody (66281-1-Ig)

    IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YY1 Monoclonal antibody (66281-1-Ig)

    IHC staining of human colon cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using YY1 Monoclonal antibody (66281-1-Ig)

    IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YY1 Monoclonal antibody (66281-1-Ig)

    IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YY1 Monoclonal antibody (66281-1-Ig)

    IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Monoclonal antibody (66281-1-Ig)

    IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66281-1-Ig (YY1 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Monoclonal antibody (66281-1-Ig)

    IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (66281-1-Ig, Clone: 2E11C5 ) at dilution of 1:1800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Monoclonal antibody (66281-1-Ig)

    IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Monoclonal antibody (66281-1-Ig)

    IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:800 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Monoclonal antibody (66281-1-Ig)

    IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66281-1-PBS targets YY1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen YY1 fusion protein Ag17732 相同性解析による交差性が予測される生物種
Full Name YY1 transcription factor
Calculated molecular weight 414 aa, 45 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC037308
Gene symbol YY1
Gene ID (NCBI) 7528
RRIDAB_2881664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.