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ZAP70 Monoclonal antibody
ZAP70 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, pig
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1A11G9
Cat no : 60200-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, pig spleen tissue, pig thymus tissue |
Positive IHC detected in | human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
60200-1-Ig targets ZAP70 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Reactivity | human, mouse, pig |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | ZAP70 fusion protein Ag7943 相同性解析による交差性が予測される生物種 |
Full Name | zeta-chain (TCR) associated protein kinase 70kDa |
Calculated molecular weight | 70 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC039039 |
Gene symbol | ZAP70 |
Gene ID (NCBI) | 7535 |
RRID | AB_10944567 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.
Protocols
Product Specific Protocols | |
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WB protocol for ZAP70 antibody 60200-1-Ig | Download protocol |
IHC protocol for ZAP70 antibody 60200-1-Ig | Download protocol |
IF protocol for ZAP70 antibody 60200-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function | ||
Cell Immunol Cbl-b inhibition promotes less differentiated phenotypes of T cells with enhanced cytokine production |