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  • Featured Product
  • KD/KO Validated

ZAP70 Monoclonal antibody, PBS Only

ZAP70 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A11G9

Cat no : 60200-1-PBS

Synonyms

70 kDa zeta associated protein, FLJ17670, FLJ17679, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    WB result of ZAP70 antibody (60200-1-Ig, 1:10000) with si-control and si-ZAP70 transfected Jurkat cells.. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of pig spleen using 60200-1-Ig (same clone as 60200-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig thymus tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of pig thymus using 60200-1-Ig (same clone as 60200-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IF Staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60200-1-Ig (ZAP70 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60200-1-PBS targets ZAP70 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag7943 相同性解析による交差性が予測される生物種
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039039
Gene symbol ZAP70
Gene ID (NCBI) 7535
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.