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  • Featured Product
  • KD/KO Validated

ZAP70 Monoclonal antibody, PBS Only (Detector)

ZAP70 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11G9

Cat no : 60200-1-PBS

Synonyms

STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein, 70 kDa zeta associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    WB result of ZAP70 antibody (60200-1-Ig, 1:10000) with si-control and si-ZAP70 transfected Jurkat cells.. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of pig spleen using 60200-1-Ig (same clone as 60200-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig thymus tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of pig thymus using 60200-1-Ig (same clone as 60200-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-1-Ig)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ZAP70 Monoclonal antibody (60200-1-Ig)

    IF Staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60200-1-Ig (ZAP70 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51134-1

    Cytometric bead array standard curve of MP51134-1

    Cytometric bead array standard curve of MP51134-1, ZAP70 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60200-3-PBS. Detection antibody: 60200-1-PBS. Standard:Ag7943. Range: 0.391-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60200-1-PBS targets ZAP70 as part of a matched antibody pair:

MP51134-1: 60200-3-PBS capture and 60200-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag7943 相同性解析による交差性が予測される生物種
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039039
Gene symbol ZAP70
Gene ID (NCBI) 7535
RRIDAB_10944567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.