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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZBTB7A Recombinant antibody (83616-5-RR)

    WB analysis using 83616-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83616-5-RR (ZBTB7A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZBTB7A Recombinant antibody (83616-5-RR)

    WB analysis of HEK-293 using 83616-5-RR

    WB result of ZBTB7A antibody (83616-5-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZBTB7A transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ZBTB7A Recombinant antibody (83616-5-RR)

    IHC staining of human stomach cancer using 83616-5-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83616-5-RR (ZBTB7A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZBTB7A Recombinant antibody (83616-5-RR)

    IF Staining of HepG2 using 83616-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZBTB7A antibody (83616-5-RR, Clone: 240555A6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZBTB7A Recombinant antibody (83616-5-RR)

    IF Staining of HepG2 using 83616-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZBTB7A antibody (83616-5-RR, Clone: 240555A6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using ZBTB7A Recombinant antibody (83616-5-RR)

    FC experiment of HeLa using 83616-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZBTB7A Recombinant antibody (83616-5-RR, Clone:240555A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • Featured Product
  • KD/KO Validated

ZBTB7A Recombinant antibody

ZBTB7A Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83616-5-RR
Clone No.240555A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

HIV 1 1st binding protein 1, FBI-1, FBI1, FBI 1, Factor binding IST protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:100-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83616-5-RR targets ZBTB7A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 7A
Calculated molecular weight 61 kDa
Observed molecular weight61 kDa, 75-80 kDa
GenBank accession numberBC113511
Gene Symbol ZBTB7A
Gene ID (NCBI) 51341
RRIDAB_3671230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95365
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZBTB7A, also named as LRF, FBI1, FBI-1, pokemon, ZBTB7, ZNF857A and DKFZp547O146, a member of the POK (POZ and Krüppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis (PMID: 35798238). ZBTB7A is involved in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. ZBTB7A is widely expressed in normal thymus and it is aberrantly overexpressed in human cancers (PMID: 28942243). ZBTB7A has 10 potential sumoylation sites located at lysine 61, 354, 371, 379, 383, 396, 486, 487, 536 and 539 (PMID: 20471975, 19471103). ZBTB7A has a predicted molecular weight of 61 kDa and the 75~80-kDa band could be the sumoylated form.

Protocols

Product Specific Protocols
WB protocol for ZBTB7A antibody 83616-5-RRDownload protocol
IHC protocol for ZBTB7A antibody 83616-5-RRDownload protocol
IF protocol for ZBTB7A antibody 83616-5-RRDownload protocol
FC protocol for ZBTB7A antibody 83616-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols