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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ZBTB7A Recombinant a (CL488-83616-5)

    IF Staining of HepG2 using CL488-83616-5

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ZBTB7A antibody (CL488-83616-5, Clone: 240555A6 ) at dilution of 1:200, CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ZBTB7A Recombinant antibody

ZBTB7A Recombinant Antibody for IF/ICC
Cat No. CL488-83616-5
Clone No.240555A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83616-5 targets ZBTB7A in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 7A
Calculated molecular weight 61 kDa
Observed molecular weight61 kDa, 75-80 kDa
GenBank accession numberBC113511
Gene Symbol ZBTB7A
Gene ID (NCBI) 51341
RRIDAB_3673283
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95365
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZBTB7A, also named as LRF, FBI1, FBI-1, pokemon, ZBTB7, ZNF857A and DKFZp547O146, a member of the POK (POZ and Krüppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis (PMID: 35798238). ZBTB7A is involved in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. ZBTB7A is widely expressed in normal thymus and it is aberrantly overexpressed in human cancers (PMID: 28942243). ZBTB7A has 10 potential sumoylation sites located at lysine 61, 354, 371, 379, 383, 396, 486, 487, 536 and 539 (PMID: 20471975, 19471103). ZBTB7A has a predicted molecular weight of 61 kDa and the 75~80-kDa band could be the sumoylated form.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ZBTB7A antibody CL488-83616-5Download protocol
Standard Protocols
Click here to view our Standard Protocols