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  • Featured Product
  • KD/KO Validated

ZC3HAV1 Monoclonal antibody, PBS Only

ZC3HAV1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10B9

Cat no : 66413-1-PBS

Synonyms

FLB6421, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    WB analysis of HEK-293 using 66413-1-Ig (same clone as 66413-1-PBS)

    WB result of ZC3HAV1 antibody (66413-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3HAV1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    WB analysis of Jurkat using 66413-1-Ig (same clone as 66413-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    WB analysis of HeLa using 66413-1-Ig (same clone as 66413-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    WB analysis of HEK-293 using 66413-1-Ig (same clone as 66413-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    IHC staining of human kidney using 66413-1-Ig (same clone as 66413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    IHC staining of human kidney using 66413-1-Ig (same clone as 66413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66413-1-Ig (ZC3HAV1 Antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZC3HAV1 Monoclonal antibody (66413-1-Ig)

    IF Staining of HepG2 using 66413-1-Ig (same clone as 66413-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66413-1-Ig(ZC3HAV1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66413-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66413-1-PBS targets ZC3HAV1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZC3HAV1 fusion protein Ag10364 相同性解析による交差性が予測される生物種
Full Name zinc finger CCCH-type, antiviral 1
Calculated molecular weight699aa,78 kDa; 902aa,101 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC025308
Gene symbol ZC3HAV1
Gene ID (NCBI) 56829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.