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ZC3HAV1 Monoclonal antibody
ZC3HAV1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1G10B9
Cat no : 66413-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
66413-1-Ig targets ZC3HAV1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ZC3HAV1 fusion protein Ag10364 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger CCCH-type, antiviral 1 |
| Calculated molecular weight | 699aa,78 kDa; 902aa,101 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC025308 |
| Gene symbol | ZC3HAV1 |
| Gene ID (NCBI) | 56829 |
| RRID | AB_2881785 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3HAV1 antibody 66413-1-Ig | Download protocol |
| IHC protocol for ZC3HAV1 antibody 66413-1-Ig | Download protocol |
| IF protocol for ZC3HAV1 antibody 66413-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
mBio The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts.
| ||
J Virol Riplet Binds the Zinc Finger Antiviral Protein (ZAP) and Augments ZAP-Mediated Restriction of HIV-1. | ||
EMBO Rep Interferon inhibits a model RNA virus via a limited set of inducible effector genes | ||
