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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ZC3HAV1 Monoclonal antibody

ZC3HAV1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10B9

Cat no : CL488-66413

Synonyms

DKFZp686F2052, DKFZp686H1869, DKFZp686O19171, FLB6421, FLJ13288, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ZC3HAV1 Monoclonal a (CL488-66413)

    IF Staining of HepG2 using CL488-66413

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66413 (ZC3HAV1 antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66413 targets ZC3HAV1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZC3HAV1 fusion protein Ag10364 相同性解析による交差性が予測される生物種
Full Name zinc finger CCCH-type, antiviral 1
Calculated molecular weight699aa,78 kDa; 902aa,101 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC025308
Gene symbol ZC3HAV1
Gene ID (NCBI) 56829
RRIDAB_2883331
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

he zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ZC3HAV1 antibody CL488-66413Download protocol
Standard Protocols
Click here to view our Standard Protocols