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- KD/KO Validated
ZFP36 Monoclonal antibody
ZFP36 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, pig
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1A5E6
Cat no : 66938-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, A549 cells, NCI-H1299 cells, LPS treated U-937 cells, 3T3-L1 cells, pig lung tissue, RAW 264.7 cells |
| Positive IHC detected in | rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
66938-1-Ig targets ZFP36 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ZFP36 fusion protein Ag28473 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 36, C3H type, homolog (mouse) |
| Calculated molecular weight | 326 aa, 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC009693 |
| Gene symbol | ZFP36 |
| Gene ID (NCBI) | 7538 |
| RRID | AB_2882262 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZFP36 antibody 66938-1-Ig | Download protocol |
| IHC protocol for ZFP36 antibody 66938-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma | ||
Virus Res ZFP36 Facilitates Senecavirus A (SVA) Replication by Inhibiting the Production of Type I Interferon
| ||
Int Immunopharmacol Angiotensin II contributes to intratumoral immunosuppressionvia induction of PD-L1 expression in non-small cell lung carcinoma.
|
