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  • Featured Product
  • KD/KO Validated

ZFP36 Monoclonal antibody, PBS Only

ZFP36 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5E6

Cat no : 66938-1-PBS

Synonyms

TTP, Zfp 36, Tristetraprolin, mRNA decay activator protein ZFP36, Growth factor-inducible nuclear protein NUP475

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZFP36 Monoclonal antibody (66938-1-Ig)

    WB analysis of HepG2 using 66938-1-Ig (same clone as 66938-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using ZFP36 Monoclonal antibody (66938-1-Ig)

    WB analysis of A549 using 66938-1-Ig (same clone as 66938-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCI-H1299 cells using ZFP36 Monoclonal antibody (66938-1-Ig)

    WB analysis of NCI-H1299 using 66938-1-Ig (same clone as 66938-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZFP36 Monoclonal antibody (66938-1-Ig)

    WB analysis using 66938-1-Ig (same clone as 66938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 3T3-L1 cells using ZFP36 Monoclonal antibody (66938-1-Ig)

    WB analysis of 3T3-L1 using 66938-1-Ig (same clone as 66938-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig lung tissue using ZFP36 Monoclonal antibody (66938-1-Ig)

    WB analysis of pig lung using 66938-1-Ig (same clone as 66938-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ZFP36 Monoclonal antibody (66938-1-Ig)

    IHC staining of rat liver using 66938-1-Ig (same clone as 66938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66938-1-Ig (ZFP36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ZFP36 Monoclonal antibody (66938-1-Ig)

    IHC staining of rat liver using 66938-1-Ig (same clone as 66938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66938-1-Ig (ZFP36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66938-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66938-1-PBS targets ZFP36 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZFP36 fusion protein Ag28473 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 36, C3H type, homolog (mouse)
Calculated molecular weight 326 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC009693
Gene symbol ZFP36
Gene ID (NCBI) 7538
RRIDAB_2882262
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF).