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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZMPSTE24 Recombinant antibody

ZMPSTE24 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241288C3

Cat no : 84547-2-RR

Synonyms

FACE-1, FACE1, FACE 1, EC:3.4.24.84, CAAX prenyl protease 1 homolog

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ZMPSTE24 Recombinant antibody (84547-2-RR)

    IF Staining of PC-3 using 84547-2-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ZMPSTE24 antibody (84547-2-RR, Clone: 241288C3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84547-2-RR

    Affinity and Kinetic Characterization of 84547-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84547-2-RR against Human ZMPSTE24 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84547-2-RR is ZMPSTE24 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZMPSTE24 fusion protein Ag33613 相同性解析による交差性が予測される生物種
Full Name zinc metallopeptidase (STE24 homolog, S. cerevisiae)
Calculated molecular weight 475 aa, 55 kDa
GenBank accession numberBC037283
Gene symbol ZMPSTE24
Gene ID (NCBI) 10269
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZMPSTE24(CAAX prenyl protease 1 homolog) gene encodes a zinc metalloproteinase involved in the processing of farnesylated proteins(PubMed: 10373325). The significance of ZMPSTE24 in human disease stems from its role as an enzyme necessary for the correct processing and maturation of lamin A ,an intermediate filament component of the nuclear envelope (PubMed: 16297189).

Protocols

Product Specific Protocols
IF protocol for ZMPSTE24 antibody 84547-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols