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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF217 Recombinant antibody (84276-1-RR)

    WB analysis using 84276-1-RR (same clone as 84276-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84276-1-RR (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01357-1

    Sandwich ELISA standard curve of MP01357-1

    Sandwich ELISA standard curve of MP01357-1, Human ZNF217 Recombinant Matched Antibody Pair - PBS only. 84276-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag19453. 84276-2-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL

  • Sample test of MP01357-1

    Sample test of MP01357-1

    The mean ZNF217 concentration was determined to be 885 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF217 Recombinant antibody (84276-1-RR)

    IF Staining of HepG2 using 84276-1-RR (same clone as 84276-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF217 Recombinant antibody (84276-1-RR)

    IF Staining of HepG2 using 84276-1-RR (same clone as 84276-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84276-1-RR

    Affinity and Kinetic Characterization of 84276-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84276-1-RR against Human ZNF217 were performed. The affinity constant is below 1 pM.

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ZNF217 Recombinant antibody, PBS Only (Capture)

ZNF217 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84276-1-PBS
Clone No.241217E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ZFP217, Zinc finger protein 217, ZABC1, 241217E3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84276-1-PBS targets ZNF217 as part of a matched antibody pair:

MP01357-1: 84276-1-PBS capture and 84276-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZNF217 fusion protein Ag19453 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 217
Calculated molecular weight 1048 aa, 115 kDa
Observed molecular weight~ 130 kDa
GenBank accession numberBC113427
Gene Symbol ZNF217
Gene ID (NCBI) 7764
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75362
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis. PMID: 38259608