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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF217 Recombinant antibody (84276-1-RR)

    WB analysis using 84276-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84276-1-RR (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF217 Recombinant antibody (84276-1-RR)

    IF Staining of HepG2 using 84276-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF217 Recombinant antibody (84276-1-RR)

    IF Staining of HepG2 using 84276-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84276-1-RR

    Affinity and Kinetic Characterization of 84276-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84276-1-RR against Human ZNF217 were performed. The affinity constant is below 1 pM.

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ZNF217 Recombinant antibody

ZNF217 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84276-1-RR
Clone No.241217E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ZFP217, Zinc finger protein 217, ZABC1, 241217E3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, LNCaP cells, Caco-2 cells, PC-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84276-1-RR targets ZNF217 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZNF217 fusion protein Ag19453 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 217
Calculated molecular weight 1048 aa, 115 kDa
Observed molecular weight~ 130 kDa
GenBank accession numberBC113427
Gene Symbol ZNF217
Gene ID (NCBI) 7764
RRIDAB_3671822
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75362
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis. PMID: 38259608

Protocols

Product Specific Protocols
WB protocol for ZNF217 antibody 84276-1-RRDownload protocol
IF protocol for ZNF217 antibody 84276-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols