Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, LNCaP cells, Caco-2 cells, PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84276-1-RR targets ZNF217 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ZNF217 fusion protein Ag19453 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 217 |
| Calculated molecular weight | 1048 aa, 115 kDa |
| Observed molecular weight | ~ 130 kDa |
| GenBank accession number | BC113427 |
| Gene Symbol | ZNF217 |
| Gene ID (NCBI) | 7764 |
| RRID | AB_3671822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O75362 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis. PMID: 38259608
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF217 antibody 84276-1-RR | Download protocol |
| IF protocol for ZNF217 antibody 84276-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |