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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ZNF217 Recombinant a (CL488-84276)

    IF Staining of HepG2 using CL488-84276

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ZNF217 antibody (CL488-84276, Clone: 241217E3 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated ZNF217 Recombinant antibody

ZNF217 Recombinant Antibody for IF/ICC
Cat No. CL488-84276
Clone No.241217E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84276 targets ZNF217 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZNF217 fusion protein Ag19453 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 217
Calculated molecular weight 1048 aa, 115 kDa
Observed molecular weight~ 130 kDa
GenBank accession numberBC113427
Gene Symbol ZNF217
Gene ID (NCBI) 7764
RRIDAB_3673351
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75362
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis. PMID: 38259608

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ZNF217 antibody CL488-84276Download protocol
Standard Protocols
Click here to view our Standard Protocols