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  • Featured Product
  • KD/KO Validated

ZO-2 Recombinant antibody

ZO-2 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230474D1

Cat no : 83111-1-RR

Synonyms

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZO-2 Recombinant antibody (83111-1-RR)

    WB analysis of HeLa using 83111-1-RR

    WB result of ZO-2 antibody (83111-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZO-2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ZO-2 Recombinant antibody (83111-1-RR)

    WB analysis using 83111-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83111-1-RR (ZO-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ZO-2 Recombinant antibody (83111-1-RR)

    IHC staining of mouse colon using 83111-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ZO-2 Recombinant antibody (83111-1-RR)

    IHC staining of mouse colon using 83111-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83111-1-RR

    Affinity and Kinetic Characterization of 83111-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83111-1-RR against Human ZO-2 were performed. The affinity constant is 30.8 pM.

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83111-1-RR targets ZO-2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZO-2 fusion protein Ag13534 相同性解析による交差性が予測される生物種
Full Name tight junction protein 2 (zona occludens 2)
Calculated molecular weight 134 kDa
Observed molecular weight ~150 kDa
GenBank accession numberBC027592
Gene symbol TJP2
Gene ID (NCBI) 9414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

Protocols

Product Specific Protocols
WB protocol for ZO-2 antibody 83111-1-RRDownload protocol
IHC protocol for ZO-2 antibody 83111-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols