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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated ZO-2 Recombinant ant (CL750-83111)

    WB analysis using CL750-83111

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83111 (ZO-2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 750-conjugated ZO-2 Recombinant ant (CL750-83111)

    FC experiment of A431 using CL750-83111

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody (CL750-83111, Clone:230474D1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody

ZO-2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-83111
Clone No.230474D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Synonyms

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-83111 targets ZO-2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZO-2 fusion protein Ag13534 相同性解析による交差性が予測される生物種
Full Name tight junction protein 2 (zona occludens 2)
Calculated molecular weight 134 kDa
Observed molecular weight ~150 kDa
GenBank accession numberBC027592
Gene Symbol TJP2
Gene ID (NCBI) 9414
RRIDAB_3673769
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UDY2
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 ZO-2 antibody CL750-83111Download protocol
FC protocol for CL Plus 750 ZO-2 antibody CL750-83111Download protocol
Standard Protocols
Click here to view our Standard Protocols