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CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody
ZO-2 Recombinant Antibody for WB, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, FC (Intra)
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
230474D1
Cat no : CL750-83111
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, MCF-7 cells |
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL750-83111 targets ZO-2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | ZO-2 fusion protein Ag13534 相同性解析による交差性が予測される生物種 |
Full Name | tight junction protein 2 (zona occludens 2) |
Calculated molecular weight | 134 kDa |
Observed molecular weight | ~150 kDa |
GenBank accession number | BC027592 |
Gene symbol | TJP2 |
Gene ID (NCBI) | 9414 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 750 ZO-2 antibody CL750-83111 | Download protocol |
FC protocol for CL Plus 750 ZO-2 antibody CL750-83111 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |