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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZP3 Recombinant antibody (85084-1-RR)

    WB analysis using 85084-1-RR (same clone as 85084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85084-1-RR (ZP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZP3 Recombinant antibody (85084-1-RR)

    WB analysis using 85084-1-RR (same clone as 85084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85084-1-RR (ZP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse ovary tissue using ZP3 Recombinant antibody (85084-1-RR)

    IF Staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP3 antibody (85084-1-RR, Clone: 242729A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse ovary tissue using ZP3 Recombinant antibody (85084-1-RR)

    IF Staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP3 antibody (85084-1-RR, Clone: 242729A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01801-2

    Cytometric bead array standard curve of MP01801-2

    Cytometric bead array standard curve of MP01801-2, ZP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85084-1-PBS. Detection antibody: 85084-2-PBS. Standard: Ag15791. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85084-1-RR

    Affinity and Kinetic Characterization of 85084-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85084-1-RR against Human ZP3 were performed. The affinity constant is below 1 pM.

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ZP3 Recombinant antibody, PBS Only (Capture)

ZP3 Uni-rAbTM Recombinant Antibody for WB, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 85084-1-PBS
Clone No.242729A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

242729A8, Processed zona pellucida sperm-binding protein 3, Zona pellucida sperm-binding protein 3, Zp 3, Zp-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85084-1-PBS targets ZP3 as part of a matched antibody pair:

MP01801-2: 85084-1-PBS capture and 85084-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZP3 fusion protein Ag15791 相同性解析による交差性が予測される生物種
Full Name zona pellucida glycoprotein 3 (sperm receptor)
Calculated molecular weight 373 aa, 41 kDa
Observed molecular weight47 kDa
GenBank accession numberBC113949
Gene Symbol ZP3
Gene ID (NCBI) 7784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21754
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that is processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).