• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ZP3 Recombinant antibody (85084-1-RR)

    WB analysis using 85084-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85084-1-RR (ZP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZP3 Recombinant antibody (85084-1-RR)

    WB analysis using 85084-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85084-1-RR (ZP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse ovary tissue using ZP3 Recombinant antibody (85084-1-RR)

    IF Staining of mouse ovary using 85084-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP3 antibody (85084-1-RR, Clone: 242729A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse ovary tissue using ZP3 Recombinant antibody (85084-1-RR)

    IF Staining of mouse ovary using 85084-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP3 antibody (85084-1-RR, Clone: 242729A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85084-1-RR

    Affinity and Kinetic Characterization of 85084-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85084-1-RR against Human ZP3 were performed. The affinity constant is below 1 pM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ZP3 Recombinant antibody

ZP3 Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA
Cat No. 85084-1-RR
Clone No.242729A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

242729A8, Processed zona pellucida sperm-binding protein 3, Zona pellucida sperm-binding protein 3, Zp 3, Zp-3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HepG2 cells, PC-3 cells
Positive IF-P detected inmouse ovary tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85084-1-RR targets ZP3 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZP3 fusion protein Ag15791 相同性解析による交差性が予測される生物種
Full Name zona pellucida glycoprotein 3 (sperm receptor)
Calculated molecular weight 373 aa, 41 kDa
Observed molecular weight47 kDa
GenBank accession numberBC113949
Gene Symbol ZP3
Gene ID (NCBI) 7784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that is processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).

Protocols

Product Specific Protocols
WB protocol for ZP3 antibody 85084-1-RRDownload protocol
IF protocol for ZP3 antibody 85084-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols