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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite®594-conjugated Zinc Alpha 2 Glycoprotein (CL594-66178)

    IF Staining of PC-3 using CL594-66178

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite®594 Zinc Alpha 2 Glycoprotein antibody (CL594-66178, Clone: 4B8A4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite®594-conjugated Zinc Alpha 2 Glycoprotein (CL594-66178)

    IF Staining of PC-3 using CL594-66178

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite®594 Zinc Alpha 2 Glycoprotein antibody (CL594-66178, Clone: 4B8A4 ) at dilution of 1:200.

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CoraLite®594-conjugated Zinc Alpha 2 Glycoprotein Monoclonal antibody

Zinc Alpha 2 Glycoprotein Monoclonal Antibody for IF/ICC
Cat No. CL594-66178
Clone No.4B8A4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

AZGP1, ZA2G, ZAG, Zinc alpha 2 glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66178 targets Zinc Alpha 2 Glycoprotein in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Zinc Alpha 2 Glycoprotein fusion protein Ag5661 相同性解析による交差性が予測される生物種
Full Name alpha-2-glycoprotein 1, zinc-binding
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC033830
Gene Symbol Zinc Alpha 2 Glycoprotein
Gene ID (NCBI) 563
RRIDAB_2934719
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP25311
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

Protocols

Product Specific Protocols
IF protocol for CL594 Zinc Alpha 2 Glycoprotein antibody CL594-66178Download protocol
Standard Protocols
Click here to view our Standard Protocols