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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Band 3/AE 1 Monoclonal antibody, PBS Only

Band 3/AE 1 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E5C1

Cat no : 66984-1-PBS

Synonyms

band 3, band 3/ AE1, SLC4A1, Anion exchanger 1, Anion exchange protein 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using Band 3/AE 1 Monoclonal antibody (66984-1-Ig)

    IHC staining of human placenta using 66984-1-Ig (same clone as 66984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66984-1-Ig (band 3/ AE1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66984-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Band 3/AE 1 Monoclonal antibody (66984-1-Ig)

    IHC staining of human placenta using 66984-1-Ig (same clone as 66984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66984-1-Ig (band 3/ AE1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66984-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Band 3/AE 1 Monoclonal antibody (66984-1-Ig)

    IF Staining of human placenta using 66984-1-Ig (same clone as 66984-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (66984-1-Ig, Clone: 1E5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66984-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66984-1-PBS targets Band 3/AE 1 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Band 3/AE 1 fusion protein Ag28058 相同性解析による交差性が予測される生物種
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Calculated molecular weight 911 aa, 102 kDa
GenBank accession numberBC101570
Gene symbol band 3
Gene ID (NCBI) 6521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)