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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Band 3/AE 1 Monoclonal antibody

Band 3/AE 1 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E5C1

Cat no : 66984-1-Ig

Synonyms

band 3, band 3/ AE1, SLC4A1, Anion exchanger 1, Anion exchange protein 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using band 3/ AE1 Monoclonal antibody (66984-1-Ig)

    IHC staining of human placenta using 66984-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66984-1-Ig (band 3/ AE1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using band 3/ AE1 Monoclonal antibody (66984-1-Ig)

    IHC staining of human placenta using 66984-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66984-1-Ig (band 3/ AE1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Band 3/ AE1 Monoclonal antibody (66984-1-Ig)

    IF Staining of human placenta using 66984-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (66984-1-Ig, Clone: 1E5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66984-1-Ig targets Band 3/AE 1 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Band 3/AE 1 fusion protein Ag28058 相同性解析による交差性が予測される生物種
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Calculated molecular weight 911 aa, 102 kDa
GenBank accession numberBC101570
Gene symbol band 3
Gene ID (NCBI) 6521
RRIDAB_2918479
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

Protocols

Product Specific Protocols
IHC protocol for Band 3/AE 1 antibody 66984-1-IgDownload protocol
IF protocol for Band 3/AE 1 antibody 66984-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Neuron

Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier

Authors - Yuan Xie
View Article