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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Band 3/AE 1 Monoclonal antibody

Band 3/AE 1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E5C1

Cat no : CL594-66984

Synonyms

band 3, band 3/ AE1, SLC4A1, Anion exchanger 1, Anion exchange protein 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CoraLite®594-conjugated Band 3/ AE1 Monoclonal ant (CL594-66984)

    IF Staining of human placenta using CL594-66984

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 band 3/ AE1 antibody (CL594-66984, Clone: 1E5C1 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66984 targets Band 3/AE 1 in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Band 3/AE 1 fusion protein Ag28058 相同性解析による交差性が予測される生物種
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Calculated molecular weight 911 aa, 102 kDa
GenBank accession numberBC101570
Gene symbol band 3
Gene ID (NCBI) 6521
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

Protocols

Product Specific Protocols
IF protocol for CL594 Band 3/AE 1 antibody CL594-66984Download protocol
Standard Protocols
Click here to view our Standard Protocols