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  • Featured Product
  • KD/KO Validated

c-Cbl Monoclonal antibody, PBS Only

c-Cbl Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F6D1

Cat no : 66576-1-PBS

Synonyms

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using c-Cbl Monoclonal antibody (66576-1-Ig)

    WB analysis using 66576-1-Ig (same clone as 66576-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66576-1-Ig (c-Cbl antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using c-Cbl Monoclonal antibody (66576-1-Ig)

    WB analysis of Daudi using 66576-1-Ig (same clone as 66576-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66576-1-Ig (CBL antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using c-Cbl Monoclonal antibody (66576-1-Ig)

    IHC staining of human tonsillitis using 66576-1-Ig (same clone as 66576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using c-Cbl Monoclonal antibody (66576-1-Ig)

    IHC staining of human tonsillitis using 66576-1-Ig (same clone as 66576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66576-1-PBS targets c-Cbl in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-Cbl fusion protein Ag23033 相同性解析による交差性が予測される生物種
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC132733
Gene symbol CBL
Gene ID (NCBI) 867
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).