Validation Data Gallery
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Tested Applications
| Positive WB detected in | HL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
66576-1-Ig targets c-Cbl in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | c-Cbl fusion protein Ag23033 相同性解析による交差性が予測される生物種 |
| Full Name | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
| Calculated molecular weight | 906 aa, 100 kDa |
| Observed molecular weight | 100-120 kDa |
| GenBank accession number | BC132733 |
| Gene Symbol | CBL |
| Gene ID (NCBI) | 867 |
| RRID | AB_2881936 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
| IHC protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.
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