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  • KD/KO Validated

c-Cbl Monoclonal antibody

c-Cbl Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66576-1-Ig
Clone No.2F6D1

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 750
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66576-1-Ig targets c-Cbl in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen c-Cbl fusion protein Ag23033 相同性解析による交差性が予測される生物種
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC132733
Gene Symbol CBL
Gene ID (NCBI) 867
RRIDAB_2881936
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22681
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Protocols

Product Specific Protocols
WB protocol for c-Cbl antibody 66576-1-IgDownload protocol
IHC protocol for c-Cbl antibody 66576-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.

Authors - Weijie Dong
  • KD Validated
humanWB

Cytokine

The CBL-LSD1-CXCL8 axis regulates methionine metabolism in glioma.

Authors - Jie Chang
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