Validation Data Gallery
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Tested Applications
| Positive WB detected in | Raji cells, mouse brain tissue, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-66576 targets c-Cbl in WB applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | c-Cbl fusion protein Ag23033 相同性解析による交差性が予測される生物種 |
| Full Name | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
| Calculated molecular weight | 906 aa, 100 kDa |
| Observed molecular weight | 100-120 kDa |
| GenBank accession number | BC132733 |
| Gene Symbol | CBL |
| Gene ID (NCBI) | 867 |
| RRID | AB_3084895 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22681 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 c-Cbl antibody CL750-66576 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |