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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using cIAP2 Recombinant antibody (84779-4-RR)

    WB analysis using 84779-4-RR (same clone as 84779-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84779-4-RR (cIAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84779-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Raji cells using cIAP2 Recombinant antibody (84779-4-RR)

    IP experiment of Raji using 84779-4-RR (same clone as 84779-4-PBS)

    IP result of anti-cIAP2 (IP:84779-4-RR, 4ug; Detection:84779-4-RR 1:1000) with Raji cells lysate 1600 ug. This data was developed using the same antibody clone with 84779-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Raji cells using cIAP2 Recombinant antibody (84779-4-RR)

    FC experiment of Raji using 84779-4-RR (same clone as 84779-4-PBS)

    1x10^6 Raji cells were intracellularly stained with 0.25 ug cIAP2 Recombinant antibody (84779-4-RR, Clone:242214E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84779-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84779-4-RR

    Affinity and Kinetic Characterization of 84779-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84779-4-RR against Human cIAP2 were performed. The affinity constant is 46.8 pM.

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cIAP2 Recombinant antibody, PBS Only

cIAP2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 84779-4-PBS
Clone No.242214E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

BIRC3, Cellular inhibitor of apoptosis 2, C IAP2, BIRC 3, Baculoviral IAP repeat-containing protein 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84779-4-PBS targets cIAP2 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen cIAP2 fusion protein Ag20500 相同性解析による交差性が予測される生物種
Full Name baculoviral IAP repeat-containing 3
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC027485
Gene Symbol cIAP2
Gene ID (NCBI) 330
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13489
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.