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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

cIAP2 Recombinant antibody

cIAP2 Recombinant Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

242214E11

Cat no : 84779-4-RR

Synonyms

BIRC3, Cellular inhibitor of apoptosis 2, C IAP2, BIRC 3, Baculoviral IAP repeat-containing protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using cIAP2 Recombinant antibody (84779-4-RR)

    WB analysis using 84779-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84779-4-RR (cIAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using cIAP2 Recombinant antibody (84779-4-RR)

    IP experiment of Raji using 84779-4-RR

    IP result of anti-cIAP2 (IP:84779-4-RR, 4ug; Detection:84779-4-RR 1:1000) with Raji cells lysate 1600 ug.

  • Flow cytometry (FC) experiment of Raji cells using cIAP2 Recombinant antibody (84779-4-RR)

    FC experiment of Raji using 84779-4-RR

    1x10^6 Raji cells were intracellularly stained with 0.25 ug cIAP2 Recombinant antibody (84779-4-RR, Clone:242214E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inDaudi cells, Raji cells
Positive IP detected inRaji cells
Positive FC (Intra) detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84779-4-RR targets cIAP2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen cIAP2 fusion protein Ag20500 相同性解析による交差性が予測される生物種
Full Name baculoviral IAP repeat-containing 3
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC027485
Gene symbol cIAP2
Gene ID (NCBI) 330
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.

Protocols

Product Specific Protocols
WB protocol for cIAP2 antibody 84779-4-RRDownload protocol
IP protocol for cIAP2 antibody 84779-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols