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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using calcyphosine Monoclonal antibody (68225-1-Ig)

    WB analysis using 68225-1-Ig (same clone as 68225-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68225-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using calcyphosine Monoclonal antibody (68225-1-Ig)

    WB analysis using 68225-1-Ig (same clone as 68225-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68225-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit lung tissue using calcyphosine Monoclonal antibody (68225-1-Ig)

    WB analysis of rabbit lung using 68225-1-Ig (same clone as 68225-1-PBS)

    rabbit lung tissue were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68225-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using calcyphosine Monoclonal antibody (68225-1-Ig)

    IF Staining of U-251 using 68225-1-Ig (same clone as 68225-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using calcyphosine antibody (68225-1-Ig, Clone: 2B7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68225-1-PBS in a different storage buffer formulation.

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calcyphosine Monoclonal antibody, PBS Only

calcyphosine Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68225-1-PBS
Clone No.2B7D1

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Calcyphosin, calcyphosine, CAPS, CAPS1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68225-1-PBS targets calcyphosine in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Reactivity Human, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen calcyphosine fusion protein Ag10330 相同性解析による交差性が予測される生物種
Full Name calcyphosine
Calculated molecular weight 189 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC080599
Gene Symbol calcyphosine
Gene ID (NCBI) 828
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13938
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.