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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

calcyphosine Monoclonal antibody

calcyphosine Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7D1

Cat no : 68225-1-Ig

Synonyms

Calcyphosin, calcyphosine, CAPS, CAPS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using calcyphosine Monoclonal antibody (68225-1-Ig)

    WB analysis using 68225-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using calcyphosine Monoclonal antibody (68225-1-Ig)

    WB analysis using 68225-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit lung tissue using calcyphosine Monoclonal antibody (68225-1-Ig)

    WB analysis of rabbit lung using 68225-1-Ig

    rabbit lung tissue were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using calcyphosine Monoclonal antibody (68225-1-Ig)

    IF Staining of U-251 using 68225-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using calcyphosine antibody (68225-1-Ig, Clone: 2B7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inSK-BR-3 cells, pig lung tissue, rabbit lung tissue, U-251 cells, PC-3 cells
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68225-1-Ig targets calcyphosine in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Reactivity Human, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen calcyphosine fusion protein Ag10330 相同性解析による交差性が予測される生物種
Full Name calcyphosine
Calculated molecular weight 189 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC080599
Gene symbol calcyphosine
Gene ID (NCBI) 828
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.

Protocols

Product Specific Protocols
WB protocol for calcyphosine antibody 68225-1-IgDownload protocol
IF protocol for calcyphosine antibody 68225-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols