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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated calcyphosine Monoclonal antibody

calcyphosine Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B7D1

Cat no : CL488-68225

Synonyms

CAPS, CAPS1, Calcyphosin, 2B7D1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using CoraLite® Plus 488-conjugated calcyphosine Monoclo (CL488-68225)

    IF Staining of U-251 using CL488-68225

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 calcyphosine antibody (CL488-68225, Clone: 2B7D1 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68225 targets calcyphosine in IF/ICC applications and shows reactivity with human, pig, rabbit samples.

Tested Reactivity human, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen calcyphosine fusion protein Ag10330 相同性解析による交差性が予測される生物種
Full Name calcyphosine
Calculated molecular weight 189 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC080599
Gene symbol calcyphosine
Gene ID (NCBI) 828
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 calcyphosine antibody CL488-68225Download protocol
Standard Protocols
Click here to view our Standard Protocols