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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

calreticulin Recombinant antibody

calreticulin Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B17

Cat no : 80017-1-RR

Synonyms

CALR, Calregulin, 2B17

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using calreticulin Recombinant antibody (80017-1-RR)

    WB analysis using 80017-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using calreticulin Recombinant antibody (80017-1-RR)

    WB analysis of NIH/3T3 using 80017-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using calreticulin Recombinant antibody (80017-1-RR)

    WB analysis of HSC-T6 using 80017-1-RR

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using calreticulin Recombinant antibody (80017-1-RR)

    IHC staining of human thyroid cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using calreticulin Recombinant antibody (80017-1-RR)

    IHC staining of human thyroid cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using calreticulin Recombinant antibody (80017-1-RR)

    IHC staining of human breast cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using calreticulin Recombinant antibody (80017-1-RR)

    IHC staining of human breast cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 80017-1-RR

    Affinity and Kinetic Characterization of 80017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80017-1-RR against Human calreticulin were performed. The affinity constant is 6.07 nM.

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman thyroid cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80017-1-RR targets calreticulin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen calreticulin fusion protein Ag26064 相同性解析による交差性が予測される生物種
Full Name calreticulin
Calculated molecular weight 60 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC002500
Gene symbol Calreticulin
Gene ID (NCBI) 811
RRIDAB_2882941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Protocols

Product Specific Protocols
WB protocol for calreticulin antibody 80017-1-RRDownload protocol
IHC protocol for calreticulin antibody 80017-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols