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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750-conjugated calreticulin Recombinant antibody

calreticulin Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2B17

Cat no : CL750-80017

Synonyms

CALR, Calregulin, 2B17

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated calreticulin Recombi (CL750-80017)

    WB analysis using CL750-80017

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-80017 (calreticulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 750-conjugated calreticulin Recombi (CL750-80017)

    FC experiment of K-562 using CL750-80017

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated calreticulin Recombinant antibody (CL750-80017, Clone:2B17)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 750-conjugated calreticulin Recombi (CL750-80017)

    FC experiment of Jurkat using CL750-80017

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated calreticulin Recombinant antibody (CL750-80017, Clone:2B17)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive FC (Intra) detected inK-562 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-80017 targets calreticulin in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen calreticulin fusion protein Ag26064 相同性解析による交差性が予測される生物種
Full Name calreticulin
Calculated molecular weight 60 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC002500
Gene symbol Calreticulin
Gene ID (NCBI) 811
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CALR, also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 calreticulin antibody CL750-80017Download protocol
Standard Protocols
Click here to view our Standard Protocols