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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated calreticulin Polyclonal antibody

calreticulin Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-27298

Synonyms

CALR, Calregulin, calreticulin, cC1qR, CRP55, CRT, CRTC, ERp60, FLJ26680, grp60, HACBP, RO, SSA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 555-conjugated calreticulin Polyclo (CL555-27298)

    IF Staining of HepG2 using CL555-27298

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 555 calreticulin antibody (CL555-27298) at dilution of 1:200, CL488-phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 555-conjugated calreticulin Polyclo (CL555-27298)

    IF Staining of HepG2 using CL555-27298

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 555 calreticulin antibody (CL555-27298) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using CoraLite® Plus 555-conjugated calreticulin Polyclo (CL555-27298)

    IF Staining of SKOV-3 using CL555-27298

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 555 calreticulin antibody (CL555-27298) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 555-conjugated calreticulin Polyclo (CL555-27298)

    FC experiment of HeLa using CL555-27298

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 555 Anti-Human calreticulin (CL555-27298) (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive IF/ICC detected inHepG2 cells, SKOV-3 cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL555-27298 targets calreticulin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen calreticulin fusion protein Ag26064 相同性解析による交差性が予測される生物種
Full Name calreticulin
Calculated molecular weight 60 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC002500
Gene symbol Calreticulin
Gene ID (NCBI) 811
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CALR, also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is an ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Protocols

Product Specific Protocols
IF protocol for CL Plus 555 calreticulin antibody CL555-27298Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biomaterials

A cuproptosis-based nanomedicine suppresses triple negative breast cancers by regulating tumor microenvironment and eliminating cancer stem cells

Authors - Chen Xiao
View Article