CoraLite® Plus 647-conjugated citrate synthase Monoclonal antibody
citrate synthase Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
2F9G6
Cat no : CL647-67784
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-67784 targets citrate synthase in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | citrate synthase fusion protein Ag9255 相同性解析による交差性が予測される生物種 |
| Full Name | citrate synthase |
| Calculated molecular weight | 466 aa, 52 kDa |
| GenBank accession number | BC010106 |
| Gene symbol | Citrate Synthase |
| Gene ID (NCBI) | 1431 |
| RRID | AB_2920310 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 citrate synthase antibody CL647-67784 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
