GFP tag Polyclonal antibody

GFP tag Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

aequorea victoria, recombinant protein and More (3)

Applications

WB, RIP, IP-MS, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 50430-2-AP

Synonyms

CFP, eGFP, eYFP, GFP, GFP tag, YFP



Tested Applications

Positive WB detected inrecombinant protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

50430-2-AP targets GFP tag in WB, RIP, IP-MS, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.

Tested Reactivity aequorea victoria, recombinant protein
Cited Reactivity rat, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFP tag fusion protein Ag2128 相同性解析による交差性が予測される生物種
Full Name GFP tag
Calculated molecular weight 26 kDa
GenBank accession numberU73901
Gene symbol
Gene ID (NCBI)
RRIDAB_11042881
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.

Protocols

Product Specific Protocols
WB protocol for GFP tag antibody 50430-2-APDownload protocol
IF protocol for GFP tag antibody 50430-2-APDownload protocol
IP protocol for GFP tag antibody 50430-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell

Phase separation drives RNA virus-induced activation of the NLRP6 inflammasome.

Authors - Chen Shen
WB,IF

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
WB

Nat Genet

Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.

Authors - Margot A Cousin
IF

Cell Stem Cell

DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State.

Authors -
WB

Nat Methods

TALEN-mediated precise genome modification by homologous recombination in zebrafish.

Authors - Zu Yao Y
IF

Immunity

Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.

Authors - Zhen Xiong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

S (Verified Customer) (05-26-2023)

Excellent

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 500
  • Cell Tissue Type: AC16
GFP tag Antibody Western Blot,Immunofluorescence validation (500 dilution) in AC16 (Cat no:50430-2-AP)
FH

PK (Verified Customer) (03-20-2023)

Very Good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: HEK
GFP tag Antibody Western Blot validation (1000 dilution) in HEK (Cat no:50430-2-AP)
FH

Stephen (Verified Customer) (08-02-2022)

HeLa cells transfected with plasmid expressing EGFP fixed with 4% PFA for 15 mins and immunostained with rabbit GFP antibody at 4 degrees incubated for 20h washed then incubated secondary alexa 594 and DAPI for 1 hour room temp imaged on wide field fluorescence microscope.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa
GFP tag Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:50430-2-AP)
FH

A (Verified Customer) (01-04-2022)

Works for IP

  • Applications: Immunoprecipitation
  • Cell Tissue Type: C. elegans
FH

Rinalda (Verified Customer) (06-21-2021)

Great product

  • Applications: Western Blot
  • Cell Tissue Type: fungal cells
FH

Lana (Verified Customer) (12-22-2020)

SDS-PAGE: 15 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) for 2h at 80V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:2500.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 – HEK293 whole cell lysate, negative transfection, 3 – whole cell lysate of cells transfected with eGFP.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: HEK293
GFP tag Antibody Western Blot validation (1:2500 dilution) in HEK293 (Cat no:50430-2-AP)
FH

Paul (Verified Customer) (01-15-2020)

Works well for WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Laura (Verified Customer) (01-14-2020)

Good sensitivity for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Aamir (Verified Customer) (01-08-2020)

Works well for WB and IF

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: HEKs
FH

Erica (Verified Customer) (09-26-2019)

This antibody works very well with both western blots and IP. We previously used GFP antibody from another company and it didn't work well. We then switched to Proteinntech's and the signal was very strong! Highly recommend!

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000 WB
  • Cell Tissue Type: HEK, MEF, HELA
FH

Mayur (Verified Customer) (04-30-2019)

The H4 cells were transfected with pcDNA only and pcDNA EGFP . The expression of the EGFP was measured using the GFP antibody from ProteinTech. Great antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5K
  • Cell Tissue Type: H4 cells
GFP tag Antibody Western Blot, validation (1:5K dilution) in H4 cells (Cat no:50430-2-AP)