CoraLite®594-conjugated GFP tag Polyclonal antibody
GFP tag Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
aequorea victoria, recombinant protein
Applications
WB, IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-50430
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
CL594-50430 targets GFP tag in WB, IF/ICC applications and shows reactivity with aequorea victoria, recombinant protein samples.
| Tested Reactivity | aequorea victoria, recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GFP tag fusion protein Ag2128 相同性解析による交差性が予測される生物種 |
| Full Name | GFP tag |
| Calculated molecular weight | 26 kDa |
| GenBank accession number | M62653 |
| Gene symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2919882 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages.
Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.
This antibody is labeled with CoraLite Plus 594.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GFP tag antibody CL594-50430 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Toxins (Basel) The Clostridium botulinum C2 Toxin Subunit C2IIa Delivers Enzymes with Positively Charged N-Termini into the Cytosol of Target Cells |
