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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

hCG Alpha Polyclonal antibody, PBS Only

hCG Alpha Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25014-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using hCG Alpha Polyclonal antibody (25014-1-AP)

    WB analysis using 25014-1-AP (same clone as 25014-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25014-1-AP (hCG alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25014-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using hCG Alpha Polyclonal antibody (25014-1-AP)

    WB analysis of human placenta using 25014-1-AP (same clone as 25014-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 25014-1-AP (hCG alpha antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Alpha Polyclonal antibody (25014-1-AP)

    IHC staining of human placenta using 25014-1-AP (same clone as 25014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25014-1-AP (hCG Alpha antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Alpha Polyclonal antibody (25014-1-AP)

    IHC staining of human placenta using 25014-1-AP (same clone as 25014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25014-1-AP (hCG Alpha antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25014-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25014-1-PBS targets hCG Alpha in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen hCG Alpha fusion protein Ag17313 相同性解析による交差性が予測される生物種
Full Name glycoprotein hormones, alpha polypeptide
Calculated molecular weight 116 aa, 13 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC020782
Gene symbol hCG Alpha
Gene ID (NCBI) 1081
RRIDAB_2879846
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.