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  • Featured Product
  • KD/KO Validated

hnRNP-E1 Polyclonal antibody, PBS Only

hnRNP-E1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14523-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    WB analysis using 14523-1-AP (same clone as 14523-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    WB analysis of HeLa using 14523-1-AP (same clone as 14523-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    WB analysis of K-562 using 14523-1-AP (same clone as 14523-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    WB analysis of MCF-7 using 14523-1-AP (same clone as 14523-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    WB analysis of HepG2 using 14523-1-AP (same clone as 14523-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    IF Staining of HepG2 using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    IF Staining of NIH/3T3 using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    IF Staining of A431 using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using hnRNP-E1 Polyclonal antibody (14523-1-AP)

    IF Staining of HeLa using 14523-1-AP (same clone as 14523-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14523-1-PBS targets hnRNP-E1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen hnRNP-E1 fusion protein Ag6007 相同性解析による交差性が予測される生物種
Full Name poly(rC) binding protein 1
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC039742
Gene symbol PCBP1
Gene ID (NCBI) 5093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

hnRNP-E1 (heterogeneous nuclear ribonucleoprotein E1) is also named as PCBP1 and Alpha-CP1. Poly(rC)-binding protein 1 (PCBP1 also called hnRNP E1) belongs to a hnRNP family that is composed of hnRNP K/J and the alpha-complex proteins (PCBP1-4α or CP1-4). Proteins in this family contain three hnRNP K homology (KH) domains for RNA-binding. The human PCBP1 gene encodes the hnRNP E1 protein, and was initially defined as clone sub2.3, with poly(C)-binding activity and observed similarity to hnRNP E2 (PMID: 8152927). hnRNP-E1 is an RNA binding protein that regulates diverse processes including translation and splicing (PMID:29396052). hnRNP E1 regulates splicing of EMT-associated genes including CD44 and PNUTS (PMID: 28825698). This antibody recognizes hnRNP E1/E2.