Validation Data Gallery
Product Information
Kit for IP and Co-IP of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)
| Description | The iST RFP-Trap® Kit for processing RFP-fusion proteins and their interacting partners for mass spectrometry analysis: The kit includes the RFP-Trap for IP/Co-IP of RFP-fusion proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:
• Reliable dentification of RFP-fusion proteins & interacting partners by MS • Identification of even low abundant RFP-fusion proteins • Superior processing of IP samples into clean peptide mixtures • Intuitive protocol for reproducible results |
| Applications | IP, MS |
| Specificity/Target | mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 22.5 μg of recombinant RFP per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 5 nM |
| Compatibility with mass spectrometry | The RFP-Trap® is optimized for on-bead digestion. |
| Storage Buffer | 20% ethanol |
| Storage Condition | Kit is shipped at ambient temperature. Upon receipt store RFP-Trap at 4°C and Digest at -20°C |
Kit components
| Component | Description |
|---|---|
| RFP-Trap Agarose | 8 reactions plus 2 controls |
| Digest | Enzyme Trypsin-mix to digest proteins. |
| Resuspend buffer | Protease reconstitution buffer for enzymes. |
| Lyse buffer | Denature, reduce and alkylate proteins. |
| Stop solution | Stop the enzymatic activity. |
| Wash 1 buffer | Clean up peptides from hydrophobic contaminants. |
| Wash 2 buffer | Clean up peptides from hydrophilic contaminants. |
| Elution buffer | Elute the peptides from the cartridge. |
| LC-Load load | Load peptides on reversed-phase LC-MS column. |
| Cartridges | Cartridge for 1 to 100 µg protein starting material. |
| Waste tubes | Tube for collecting waste after washing steps. |
| Collection tubes | Tube for collecting peptides after elution. |
| Adapters | Enables placing a cartridge into a tube. |
| Caps | Cap to optionally close the cartridge’s bottom. |
Documentation
| SDS |
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| SDS iST Kits (grouped) EtOH (EN)-CTK |
| Datasheet |
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| Protocol rtak-iST-iST RFP-Trap Kit (PDF) |
| RFP-Trap Specificity |
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| Fluorescent protein specificity table (PDF) |