Ki-67 Polyclonal antibody
Ki-67 Polyclonal Antibody for IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse and More (2)
Applications
WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment
Conjugate
Unconjugated
Cat no : 28074-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse colon tissue, mouse spleen tissue |
| Positive IF-Fro detected in | mouse spleen tissue |
| Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:1500-1:6000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 16 publications below |
| IHC | See 49 publications below |
| IF | See 14 publications below |
Product Information
28074-1-AP targets Ki-67 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Ki-67 fusion protein Ag27894 相同性解析による交差性が予測される生物種 |
| Full Name | antigen identified by monoclonal antibody Ki 67 |
| Calculated molecular weight | 351 kDa |
| GenBank accession number | NM_001081117 |
| Gene symbol | ki67 |
| Gene ID (NCBI) | 17345 |
| RRID | AB_2918145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Ki-67 antibody 28074-1-AP | Download protocol |
| IF protocol for Ki-67 antibody 28074-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Lansoprazole (LPZ) reverses multidrug resistance (MDR) in cancer through impeding ATP-binding cassette (ABC) transporter-mediated chemotherapeutic drug efflux and lysosomal sequestration | ||
Cell Mol Immunol Targeting PHGDH reverses the immunosuppressive phenotype of tumor-associated macrophages through α-ketoglutarate and mTORC1 signaling | ||
Biosens Bioelectron 3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing | ||
Adv Healthc Mater A Multifunctional Nanodrug Increases the Therapeutic Sensitivity of Lenvatinib to Hepatocellular Carcinoma by Inhibiting the Stemness of Hepatic Cancer Stem Cells | ||
Cancer Lett Icaritin with autophagy/mitophagy inhibitors synergistically enhances anticancer efficacy and apoptotic effects through PINK1/Parkin-mediated mitophagy in hepatocellular carcinoma | ||
ACS Appl Mater Interfaces Doxorubicin-Loaded Microalgal Delivery System for Combined Chemotherapy and Enhanced Photodynamic Therapy of Osteosarcoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ruchi (Verified Customer) (06-08-2023) | Stained Ki67 on murine diabetic skin
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