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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody

Ki-67 Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-28074

Synonyms

Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using CoraLite® Plus 647-conjugated ki67 Polyclonal anti (CL647-28074)

    IF Staining of mouse colon using CL647-28074

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using CoraLite® Plus 647-conjugated ki67 Polyclonal anti (CL647-28074)

    IF Staining of mouse colon using CL647-28074

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated ki67 Polyclonal anti (CL647-28074)

    IF Staining of HeLa using CL647-28074

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse colon tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-28074 targets Ki-67 in IF/ICC, IF-P applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ki-67 fusion protein Ag27894 相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki 67
Calculated molecular weight 351 kDa
GenBank accession numberNM_001081117
Gene symbol ki67
Gene ID (NCBI) 17345
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Ki-67 antibody CL647-28074Download protocol
Standard Protocols
Click here to view our Standard Protocols