mCherry Monoclonal antibody
mCherry Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (1)
Applications
WB, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1B3C6
Cat no : 68088-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
68088-1-Ig targets mCherry in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | mCherry fusion protein Ag25320 相同性解析による交差性が予測される生物種 |
| Full Name | mCherry |
| Calculated molecular weight | 27 kDa |
| Gene symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918825 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for mCherry antibody 68088-1-Ig | Download protocol |
| IF protocol for mCherry antibody 68088-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Open Biol Coiled-coil-mediated dimerization of Atg16 is required for binding to the PROPPIN Atg21 | ||
J Biol Chem Porcine IKKε is involved in the STING-induced type I IFN antiviral response of the cytosolic DNA signaling pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christine (Verified Customer) (10-02-2024) | We are running out of a home-made sheep anti RFP antibody and trying to find a replacement. I ran samples transfected with a mCHERRY-tagged protein side by side to compare our old antibody and this one. Although I detected clearly the expression of the mCHERRY tagged protein with this antibody, it was not as sensitive as the sheep one we had and there were some bands of my protein of interest that I could not detect clearly. Upping the concentration from 1:5,000 to 1:1,000 did not make any clear difference. So although the antibody works it is not sensitive enough for my needs.
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