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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using mCherry Monoclonal antibody (68088-1-Ig)

    WB analysis of HEK-293 using 68088-1-Ig (same clone as 68088-1-PBS)

    Non-transfected and transfected HEK-293 lysates were subjected to SDS PAGE followed by western blot with 68088-1-Ig (mCherry antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68088-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using mCherry Monoclonal antibody (68088-1-Ig)

    IF Staining of Transfected HEK-293 using 68088-1-Ig (same clone as 68088-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (68088-1-Ig, Clone: 1B3C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68088-1-PBS in a different storage buffer formulation.

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mCherry Monoclonal antibody, PBS Only

mCherry Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68088-1-PBS
Clone No.1B3C6

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IF/ICC, ELISA

Synonyms

mCherry

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68088-1-PBS targets mCherry in WB, IF/ICC, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen mCherry fusion protein Ag25320 相同性解析による交差性が予測される生物種
Full Name mCherry
Calculated molecular weight 27 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.