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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using mTOR Polyclonal antibody (28273-1-AP)

    WB analysis of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    WB result of mTOR antibody (28273-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using mTOR Polyclonal antibody (28273-1-AP)

    WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28273-1-AP (mTOR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using mTOR Polyclonal antibody (28273-1-AP)

    WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    Non-treated and Rapamycin treated lysates were subjected to SDS PAGE followed by western blot with 28881-1-AP (Phospho-mTOR (Ser2448) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (28273-1-AP) and Vinculin (66305-1-Ig) subsequently. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using mTOR Polyclonal antibody (28273-1-AP)

    WB analysis of MCF-7 using 28273-1-AP (same clone as 28273-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28273-1-AP (MTOR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using mTOR Polyclonal antibody (28273-1-AP)

    WB analysis of HepG2 using 28273-1-AP (same clone as 28273-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28273-1-AP (MTOR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using mTOR Polyclonal antibody (28273-1-AP)

    WB analysis of PC-3 using 28273-1-AP (same clone as 28273-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 28273-1-AP (MTOR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using mTOR Polyclonal antibody (28273-1-AP)

    WB analysis using 28273-1-AP (same clone as 28273-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28273-1-AP (MTOR antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using mTOR Polyclonal antibody (28273-1-AP)

    IP experiment of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    IP result of anti-mTOR (IP:28273-1-AP, 4ug; Detection:28273-1-AP 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using mTOR Polyclonal antibody (28273-1-AP)

    IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using mTOR Polyclonal antibody (28273-1-AP)

    IHC staining of human prostate cancer using 28273-1-AP (same clone as 28273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using mTOR Polyclonal antibody (28273-1-AP)

    IHC staining of human breast cancer using 28273-1-AP (same clone as 28273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using mTOR Polyclonal antibody (28273-1-AP)

    IHC staining of human breast cancer using 28273-1-AP (same clone as 28273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using mTOR Polyclonal antibody (28273-1-AP)

    IF Staining of HeLa using 28273-1-AP (same clone as 28273-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using mTOR antibody (28273-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using mTOR Polyclonal antibody (28273-1-AP)

    IF Staining of HepG2 using 28273-1-AP (same clone as 28273-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (28273-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28273-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

mTOR Polyclonal antibody, PBS Only

mTOR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 28273-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28273-1-PBS targets mTOR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen mTOR fusion protein Ag28395 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight 289 kDa
Observed molecular weight 250-289 kDa
GenBank accession numberNM_004958
Gene Symbol mTOR
Gene ID (NCBI) 2475
RRIDAB_2881102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42345
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).