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p115, USO1 Monoclonal antibody

p115, USO1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B7D8

Cat no : 68100-1-Ig

Synonyms

P115, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p115, USO1 Monoclonal antibody (68100-1-Ig)

    WB analysis using 68100-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68100-1-Ig (p115, USO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Western Blot (WB) analysis of HeLa cells using p115, USO1 Monoclonal antibody (68100-1-Ig)

    WB analysis of HeLa using 68100-1-Ig

    WB result of p115, USO1 antibody (68100-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p115, USO1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using p115, USO1 Monoclonal antibody (68100-1-Ig)

    IHC staining of human liver cancer using 68100-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68100-1-Ig (p115, USO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using p115, USO1 Monoclonal antibody (68100-1-Ig)

    IHC staining of human liver cancer using 68100-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68100-1-Ig (p115, USO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using p115, USO1 Monoclonal antibody (68100-1-Ig)

    IF Staining of HeLa using 68100-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (68100-1-Ig, Clone: 3B7D8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68100-1-Ig targets p115, USO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen p115, USO1 fusion protein Ag5543 相同性解析による交差性が予測される生物種
Full Name USO1 homolog, vesicle docking protein (yeast)
Calculated molecular weight 962 aa, 108 kDa
Observed molecular weight108 kDa
GenBank accession numberBC032654
Gene symbol USO1
Gene ID (NCBI) 8615
RRIDAB_2918835
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

Protocols

Product Specific Protocols
WB protocol for p115, USO1 antibody 68100-1-IgDownload protocol
IHC protocol for p115, USO1 antibody 68100-1-IgDownload protocol
IF protocol for p115, USO1 antibody 68100-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols