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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated p115, USO1 Monoclona (CL488-68100)

    IF Staining of HeLa using CL488-68100

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 p115, USO1 antibody (CL488-68100, Clone: 3B7D8 ) at dilution of 1:100.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated p115, USO1 Monoclonal antibody

p115, USO1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68100
Clone No.3B7D8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

P115, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68100 targets p115, USO1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen p115, USO1 fusion protein Ag5543 相同性解析による交差性が予測される生物種
Full Name USO1 homolog, vesicle docking protein (yeast)
Calculated molecular weight 962 aa, 108 kDa
Observed molecular weight108 kDa
GenBank accession numberBC032654
Gene Symbol USO1
Gene ID (NCBI) 8615
RRIDAB_3084400
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60763
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 p115, USO1 antibody CL488-68100Download protocol
Standard Protocols
Click here to view our Standard Protocols