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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated p115, USO1 Polyclonal antibody

p115, USO1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-13509

Synonyms

P115, p115, USO1, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated p115, USO1 Polyclonal anti (CL594-13509)

    IF Staining of HeLa using CL594-13509

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 p115, USO1 antibody (CL594-13509) at dilution of 1:200, 488-phalloidin (green).

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-13509 targets p115, USO1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p115, USO1 fusion protein Ag4431 相同性解析による交差性が予測される生物種
Full Name USO1 homolog, vesicle docking protein (yeast)
Calculated molecular weight 962 aa, 108 kDa
GenBank accession numberBC032654
Gene symbol USO1
Gene ID (NCBI) 8615
RRIDAB_2919797
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

Protocols

Product Specific Protocols
IF protocol for CL594 p115, USO1 antibody CL594-13509Download protocol
Standard Protocols
Click here to view our Standard Protocols