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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p300 Polyclonal antibody (20695-1-AP)

    WB analysis using 20695-1-AP (same clone as 20695-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using p300 Polyclonal antibody (20695-1-AP)

    WB analysis using 20695-1-AP (same clone as 20695-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using p300 Polyclonal antibody (20695-1-AP)

    WB analysis using 20695-1-AP (same clone as 20695-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using p300 Polyclonal antibody (20695-1-AP)

    WB analysis using 20695-1-AP (same clone as 20695-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using p300 Polyclonal antibody (20695-1-AP)

    IHC staining of mouse lung using 20695-1-AP (same clone as 20695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using p300 Polyclonal antibody (20695-1-AP)

    IHC staining of mouse lung using 20695-1-AP (same clone as 20695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using p300 Polyclonal antibody (20695-1-AP)

    IHC staining of rat lung using 20695-1-AP (same clone as 20695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20695-1-PBS in a different storage buffer formulation.

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p300 Polyclonal antibody, PBS Only

p300 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20695-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EP300, Histone crotonyltransferase p300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

It is recommended to use NETN lysis buffer for sample preparation during WB detection.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20695-1-PBS targets p300 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name E1A binding protein p300
Calculated molecular weight 264 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_001429
Gene Symbol EP300
Gene ID (NCBI) 2033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ09472
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).