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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using p300 Recombinant antibody (83078-4-RR)

    IF Staining of HepG2 using 83078-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-4-RR, Clone: 230369H8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of THP-1 cells using p300 Recombinant antibody (83078-4-RR)

    FC experiment of THP-1 using 83078-4-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug p300 Recombinant antibody (83078-4-RR, Clone:230369H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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p300 Recombinant antibody

p300 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83078-4-RR
Clone No.230369H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83078-4-RR targets p300 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=1.30 x 10-8M
KOff=3.14 x 10-3M
KOn=2.42 x 105M
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name E1A binding protein p300
Calculated molecular weight 264 kDa
GenBank accession numberNM_001429
Gene Symbol EP300
Gene ID (NCBI) 2033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09472
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

Protocols

Product Specific Protocols
IF protocol for p300 antibody 83078-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols