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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

p38 MAPK Recombinant antibody, PBS Only

p38 MAPK Recombinant Antibody for WB, IP, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230263H7

Cat no : 80821-3-PBS

Synonyms

CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using p38 MAPK Recombinant antibody (80821-3-RR)

    WB analysis of mouse brain using 80821-3-RR (same clone as 80821-3-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 80821-3-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using p38 MAPK Recombinant antibody (80821-3-RR)

    IP experiment of HEK-293 using 80821-3-RR (same clone as 80821-3-PBS)

    IP result of anti-p38 MAPK (IP:80821-3-RR, 4ug; Detection:80821-3-RR 1:2000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using p38 MAPK Recombinant antibody (80821-3-RR)

    FC experiment of HeLa using 80821-3-RR (same clone as 80821-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using p38 MAPK Recombinant antibody (80821-3-RR)

    FC experiment of Jurkat using 80821-3-RR (same clone as 80821-3-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80821-3-PBS targets p38 MAPK in WB, IP, FC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen p38 MAPK fusion protein Ag5115 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 14
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight38-42 kDa
GenBank accession numberBC031574
Gene symbol MAPK14
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.